Starch-based hydrogels were performed by Diels-Alder cross-linking reactions between furan-modified starch and a water soluble bismaleimide, with improving conducing properties by using graphene layers as active nanofillers. The characterization results demonstrated that the Diels-Alder reaction and the corresponding conditions for the hydrogel formation were appropriate. The effect of increasing the furan/maleimide ratio on the architecture of the hydrogels and on the morphological, rheological and swelling properties were thoroughly evaluated. Effective network structure was obtained by increasing the cross-linker content leading to decreasing pore size and increasing storage modulus value of the final material. It was shown that the swelling behavior of hydrogels was mainly governed by the hydrophilic character of bismaleimide. Graphene nanosheets were added for the synthesis of nanocomposite hydrogel and it was characterized in terms of rheological properties, electrical conductivity and antimicrobial activity. The nanocomposite hydrogel presented enhanced mechanical performance, antimicrobial activity and increased conductivity values, up to a decade, indicating that conducive and active hydrogels could be satisfactory obtained, for a large range of potential applications such as biomed.