About six million years ago, the Mediterranean Sea underwent a period of isolation from the ocean and widespread salt deposition known as the Messinian Salinity Crisis (MSC), allegedly leading to a kilometer-scale level drawdown by evaporation. One of the competing scenarios proposed for the termination of this environmental crisis 5.3 million years ago consists of a megaflooding event refilling the Mediterranean Sea through the Strait of Gibraltar: the Zanclean flood. The main evidence supporting this hypothesis is a nearly 390 km long and several hundred meters deep erosion channel extending from the Gulf of Cadiz (Atlantic Ocean) to the Algerian Basin (Western Mediterranean), implying the excavation of ca. 1000 km(3) of Miocene sediment and bedrock. Based on the understanding obtained from Pleistocene onshore megaflooding events and using ad-hoc hydrodynamic modeling, here we explore two predictions of the Zanclean outburst flood hypothesis: 1) The formation of similar erosion features at sills communicating sub-basins within the Mediterranean Sea, specifically at the Sicily Sill; and 2) the accumulation of the eroded materials as megaflood deposits in areas of low flow energy. Recent data show a 6-km-wide amphitheater-shaped canyon preserved at the Malta Escarpment that may represent the erosional expression of the Zanclean flood after filling the western Mediterranean and spilling into the Eastern Basin. Next to that canyon, a similar to 1600 km(3) accumulation of chaotic, seismically transparent sediment has been found in the Ionian Sea, compatible in age and facies with megaflood deposits. Another candidate megaflood deposit has been identified in the Alboran Sea in the form of elongated sedimentary bodies that parallel the flooding channel and are seismically characterized by chaotic and discontinuous stratified reflections, that we interpret as equivalent to gravel and boulder megabars described in terrestrial megaflood settings. Numerical model predictions show that sand deposits found at the Miocene/Pliocene (M/P) boundary in ODP sites 974 and 975 (South Balearic and Tyrrhenian seas) are consistent with suspension transport from the Strait of Gibraltar during a flooding event at a peak water discharge of similar to 10(8) m(3) s(-1).